The Viruses- classification, structure, and diseases

Viruses are sub-microscopic, acellular, and non-cytoplasmic infectious agents. These replicates are only in the living cell of an organism. Viruses can infect every type of life form like animals, plants, microorganisms like bacteria, etc. and these are transmissible from diseased to healthy organisms.

Dmitri Ivanvosky discovered the virus (1892) in an extract of a tobacco plant that was infected with the Tobacco mosaic virus (TMV).

  • The study of Viruses is known as virology (under microbiology)
  • The term virus coined by Pasteur (Latin Virus- Venom)
  • The latest definition of a virus is given as “Viruses are infective nucleoproteins”.
General Characteristics of viruses:
  • All Viruses are obligate parasites and they can multiply only within the host cell.
  • They contain only a single nucleic acid either DNA or RNA.
  • The viruses are host-specific.
  • The viruses are found in almost every ecosystem on earth and these are the most numerous type of biological entities.
Non-living characters of viruses:
  • No protoplasm is found
  • enzymes are also not present
  • respiration does not take place
  • They can be crystalized
  • In culture medium, they cannot grow.
Living character of viruses:
  • They possess nucleic acid by this they get capable to synthesize protein.
  • they multiply inside the living cell
  • cause diseases.

on the basis of these characters, it can be said that viruses are transitional groups between living and non-living.

Classification:

Viruses can be classified on the basis of phenotype characters a morphology, nucleic acid, mode of replication, the host organism, and type of disease caused by them. Formally the viruses are classified by the ICTV (International committee on taxonomy of viruses) system. Mostly Baltimore classification system used, through it viruses placed in seven groups on the basis of the pattern of RNA synthesis.

Baltimore Classification (1971):

In this classification system viruses are placed in seven groups on the basis of nucleic acid (DNA or RNA), single-stranded or double-stranded, sense, and the method of replication. The classification is named after the Nobel prize-winning biologist David Baltimore. The seven groups are following:

  • I: dsDNA viruses (e.g. Adenoviruses, Herpesviruses, Poxviruses)
  • II: ssDNA viruses (+ strand or “sense”) DNA (e.g. Parvoviruses)
  • III: dsRNA viruses (e.g. Reoviruses)
  • IV: (+)ssRNA viruses (+ strand or sense) RNA (e.g. Coronaviruses, Picornaviruses, Togaviruses)
  • V: (−)ssRNA viruses (− strand or antisense) RNA (e.g. Orthomyxoviruses, Rhabdoviruses)
  • VI: ssRNA-RT viruses (+ strand or sense) RNA with DNA intermediate in life-cycle (e.g. Retroviruses)
  • VII: dsDNA-RT viruses DNA with RNA intermediate in life-cycle (e.g. Hepadnaviruses)

The DNA viruses: The viruses having DNA as genome (exception-  DNA reverse transcribing viruses).

  • In this type viruses with Double-Stranded DNA- a group I (e.g. chickenpox and herpes) and single-stranded DNA –group II are found.

The RNA viruses: these consisting the RNA genome.

  • The viruses of group III with a double-stranded RNA genome. e,g, Rotavirus.
  • The viruses containing positive-sense single-stranded RNA genomes (group IV virus). e.g. Hepatitis A virus, rhinoviruses, poliovirus, foot and mouth virus.
  • The group V viruses- negative-sense single-stranded RNA genome. e.g. Ebola, Marburg viruses.

Reverse transcribing viruses: These viruses encode reverse transcriptase.

  • Group VI viruses with single-stranded RNA viruses replicate through DNA intermediate. e.g. Retrovirus (HIV is a member of this gr.)
  • The viruses of group VII with double-stranded DNA genomes replicates using reverse transcriptase. e.g. Hepatitis B virus.

The Holmes Classification (1948): Holmes classified viruses into three groups as follows:

  1. Phaginae (the viruses that attack bacteria)
  2. Phytophaginae (attacks plants)
  3. Zoophaginae ( attacks animals)

This system was not accepted as morphological similarities were neglected in it.

The general structure of viruses:

Viruses are inert outside the host cell. Small viruses, e.g., polio and tobacco mosaic virus, can even be crystallized. Viruses are unable to generate energy. As obligate intracellular parasites, during replication, they fully depend on the complicated biochemical machinery of eukaryotic or prokaryotic cells. The main purpose of a virus is to deliver its genome into the host cell to allow its expression (transcription and translation) by the host cell.

The infectious virus is called a virion. The simplest virions consist of two basic components:

  1. nucleic acid (single- or double-stranded RNA or DNA) : either DNA or RNA
  2. Capsid – A protein coat, which functions as a shell to protect the viral genome from nucleases and which during infection attaches the virion to specific receptors exposed on the prospective host cell
    • Capsids are formed as single or double protein (capsomeres) shells and consist of only one or a few structural protein species.
    • Therefore, multiple protein copies must self-assemble to form the continuous three-dimensional capsid structure. The self-assembly of virus capsids follows two basic patterns one is the helical symmetry, in which the protein subunits and the nucleic acid are arranged in a helix, and second icosahedral symmetry, in which the protein subunits assemble into a symmetric shell that covers the nucleic acid-containing core.
    • Envelop: Some large virions possess glycoprotein envelop surrounding the capsid called envelopes. It is made up of two lipid layers (lipoprotein bilayer) that may contain material of host membrane or its own, it is not found in all viruses. The viruses are called enveloped e.g. influenza
Structure of TMV (Tobacco mosaic virus): (The bacterial virus)

It is a plant virus, a Tobacco mosaic virus. Ivanoski reported in 1892 that extracts from infected leaves were still infectious after filtration through a Chamberland filter candle.

Beijerinck, in 1898, was the first to call ‘virus’, the incitant of the tobacco mosaic. He showed that the incitant was able to migrate in an agar gel, therefore being an infectious soluble agent, or a ‘contagium vivum fluidum’ and definitively not a ‘contagium fixum’ as would be a bacterium

TMV virus

  • It is rod-shaped, approx. 300nm in length and ~18 nm in diameter.
  • Made up of RNA and Proteins.
  • The RNA is single-stranded, helical, coiled around the hollow axis of the rod.
  • The capsid or protein coat is made up of 2130 molecules of the coat protein.
  • The TMV is of naked type, i.e. envelop is not found, the capsid is not surrounded by an envelope.
Structure of bacteriophage:

These viruses are intracellular parasites of bacteria, so named on it, bacteriophages. In the 20th century, Fredrick Twort (an English bacteriologist) discovered viruses that infect bacteria. Later named as bacteriophages.

Bacteriophage T4 is one of the seven Escherichia coliphages (T1–T7, T for type), which, in 1944, were suggested by Delbruck and coworkers to be models for study by the phage community

Bacteriophage T4 is classified as a member in the Myoviridae family of the Caudovirales order because it has a contractile tail.

  • These may have DNA or RNA, the shape of capsid varies as isohedral, filamentous, head-tail shaped.
  • T- series bacteriophage (T3, T4, T6, etc) are most studies, these are characterized by the presence of a tail.

bacteriophase- The Viruses- classification, structure and diseases

  • They consist polyhedral head, short collar, and a helical structure tail.
    • Head: it consists of about 2000 capsomeres with double-stranded DNA. the diameter of the head is 650 A.
    • Tail: The 925 Å-long tail is surrounded by the contractile sheath and ends with a hexagonal base plate. Both tube and sheath are attached to the dome-shaped baseplate at the end away from the head. These tail fibers help bacteriophages for attaching to bacteria. These together form the baseplate that then nucleates the assembly of the tail tube around which the sheath assembles. The tail is entirely made up of proteins.

 

life cycle of bacteriophage

 

The life cycle of bacteriophages:

from the initial stage of infection of bacteria to killing bacteria for releasing virus’s progeny goes through certain stages given below:

Adsorption: attachment of viruses’ tail fibers to a specific receptor on the surface of the bacterial cell.

Penetration:  The muramidase of phage works for the weakening of the cell wall and hollow-core penetrated through it, by the process of the DNA injected inside the bacterial cell.

Multiplication or synthesize:

The Phage DNA replicates and also synthesizes new proteins for its capsid. The subunits of head, tail, and protein appear. some specific enzymes work in synthesis known as early proteins.

Maturation and assembly of phage’s

The head and tail on maturation assemble, phage DNA is surrounded by the protein coat. Formation of virion takes place by addition of tail.

Lysis:

The bacterial cell bursts by the process called lysis and new phage particles are liberated. These particles are able to infect other bacteria of the same type.

Examples of some viruses with the type of nucleic acid:
Virus Type of nucleic acid Virus Type of nucleic acid
Herpes dsDNA Measles ssRNA
Chickenpox dsDNA Mumps ssRNA
Hepatitis B dsDNA Polio ssRNA
Cyanophages dsDNA TMV ssRNA
Influenza ssRNA Mycophase dsRNA
Rabies ssRNA Reovirus dsRNA
HIV ssRNA Wound tumor virus dsRNA
Plant disease by Viruses:
Diseases Causing agent
Tobacco mosaic TMV
Papaya mosaic Papaya mosaic virus
Cucumber mosaic Cucumber mosaic virus
Potato mild mosaic Potato virus X
Potato rugose mosaic Potato virus Y
Potato leaf curl Potato leaf curl virus
Potato leaf roll Potato leafroll virus
Little leaf of brinjal Brinjal little leaf virus
Rosette of groundnut Groundnut mosaic virus
Sugar can mosaic Sugarcan (sacchraum ) virus
Animal diseases by Viruses:
Diseases Causal agent
Common cold Rhinovirus
Influenza Influenza virus
Rubella (measles) Rubella virus
poliomyelitis Poliovirus
Smallpox Variola virus
Yellow fever arbovirus
Chickenpox Varicella virus
AIDS HIV

 

The common route through with viral infection takes place in humans and name of viruses:

Through respiratory route (i.e., droplet, aerosol, and respiratory secretions on the hands and elsewhere; oral exchange): influenza virus, varicella-zoster virus, human rhinovirus, human adenovirus, respiratory syncytial virus, parainfluenza virus, metapneumovirus

By fecal or oral route: polioviruses, coxsackieviruses, hepatitis A virus, rotavirus, astrovirus, norovirus

By direct contact with infected: human papillomavirus (HPV), molluscum contagiosum, herpes simplex virus type 1 (HSV-1)

Through sexual transmission: human immunodeficiency virus type 1 (HIV-1), human T-lymphotropic virus type 1 (HTLV-1), hepatitis B virus (HBV), human papillomavirus types 16 and 18 (HPV-16, HPV18), HSV-2

Urine-associated: cytomegalovirus (CMV)

By parental route (i.e., blood and blood products, transplantation, tattooing, and scarification): HIV-1, HBV, hepatitis C virus (HCV)

Bite of animals: rabies virus, Duvenhage virus

Vertical route (e.g., germline, intrauterine, perinatal, human milk): HIV-1, HTLV-1, germline transmission of endogenous retroviruses

Arthropod-borne route (e.g., mosquitos, ticks, sandflies): Japanese encephalitis virus, West Nile virus, dengue virus, yellow fever virus, Zika virus, chikungunya virus, and many others

transmission associated with rodents: Lassa fever virus, sin Nombre, and other hantaviruses (e.g., Hantaan virus, Seoul virus, and Puumala virus)

transmission associated with bats: rabies virus, Nipah virus, Ebola virus, severe acute respiratory syndrome coronavirus (SARS CoV)

Monkey-associated transmission: herpes B virus, monkeypox virus, orf virus

Other zoonotic associations (e.g., cows, sheep): orf virus, cowpox virus

 

You can also read: Common human diseases 

References: https://www.ncbi.nlm.nih.gov/books/NBK8174/

 

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